Optimization the Mixing Ratio of Leaf and Seed Extracts of Brassica juncea L. Czern. as a Cosmetic Material

Purpose: This study aimed to investigate the physiological activity of mustard (Brassica juncea L. Czern.) leaf and seed extracts as a cosmetic material, optimization mixing ratio using response surface methodology (RSM).Methods: Optimal conditions for RSM were determined (X1) (X2) independent variable, total polyphenol content, flavonoid DPPH radical scavenging activity, ABTS tyrosinase inhibitory elastase was dependent variable.Results: with maximum antioxidant, whitening, anti-wrinkle effects 3.28 (4.10 mg/mL) leaves 4.21 (5.26 seeds. The activities, activities predicted be 80.01%, 58.50%, 22.11%, 53.88%, respectively. In addition, when mixture treated in LPS-induced RAW264.7 cells, NO production inhibited by about 41% compared control group, no cytotoxicity observed.Conclusion: this study, optimal anti-inflammatory has been confirmed, finding suggest that can use material.